Platelet lysate: a replacement for fetal bovine serum in animal cell culture?
نویسندگان
چکیده
منابع مشابه
Platelet Rich in Growth Factors (PRGF): A Suitable Replacement for Fetal Bovine Serum (FBS) in Mesenchymal Stem Cell Culture
Background: Due to high differentiation potential and self-renewality, Mesenchymal Stem Cells are now widely considered by researchers in several diseases. FBS is used as a supplement in culture media for proliferation, differentiation, and other culture processes of MSCs, which is associated with transmission risk of a variety of infections as well as immune responses. PRGF derived from platel...
متن کاملhuman autologous serum as a substitute for fetal bovine serum in human schwann cell culture.
nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. the number of studies on the role of schwann cells (sc) in treating nervous disorders is higher than other cell types. different protocols have been suggested for isolation and expansion of sc which most of them have used multiple growth factors, mitogens and fetal b...
متن کاملHuman-derived alternatives to fetal bovine serum in cell culture.
OBJECTIVE The need for an alternative to fetal bovine serum (FBS) is known to scientists and users involved in cell therapy or advanced therapy medicinal products. Human serum (huS) and platelet lysate (hPL) can be used as alternatives resulting in similar or even superior results concerning cell expansion. METHODS We developed protocols for the production of huS and two types of hPL and test...
متن کاملAlternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
The search for alternatives to the use of fetal bovine serum (FBS) in cell and tissue culture media has become a major goal in terms of the 3R principles in order to reduce or to avoid harvesting of FBS from bovine fetuses, and, in terms of Good Manufacturing Practice (GMP), to ensure safe and animal product-free conditions for biomedical tissue engineering and (adult) stem cell therapy, respec...
متن کاملHuman autologous serum as a substitute for fetal bovine serum in human Schwann cell culture.
Nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. The number of studies on the role of Schwann cells (SC) in treating nervous disorders is higher than other cell types. Different protocols have been suggested for isolation and expansion of SC which most of them have used multiple growth factors, mitogens and fetal b...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2003
ISSN: 0920-9069
DOI: 10.1023/b:cyto.0000009820.72920.cf